Version: 4.3

I did not understand the option --help Usage is 'melting OPTIONS' where OPTIONS are: -A[xxxxxx.nn] Name of a file containing alternative nn parameters Defaults are: DNA/DNA: all97a.nn DNA/RNA: sug95a.nn RNA/RNA: xia98a.nn -D[xxxxxx.nn] Name of a file containing nn parameters for dangling ends Default is dnadnade.nn -C[XXXXXXXXXX] Complementary sequence, mandatory if mismaches -F[x.xx] Correction for the concentration of nucleic acid Default is DEFAULT_NUC_CORR -h Displays this help and quit -H[xxxxxx] Type of hybridisation (exemple dnadna), mandatory -I[XXXXXX] Name of an input file setting up the options -K Salt correction. Default is san98a -L Displays legal information and quit -M[xxxxxx.nn] Name of a file containing nn parameters for mismatches Default is dnadnamm.nn -i[xxxxxx.nn] Name of a file containing nn parameters for inosine mismatches Defaults are: DNA/DNA: san05a.nn DNA/RNA: san05a.nn RNA/RNA: bre07a.nn -N[x.xe-x] Sodium concentration in mol.l-1. Mandatory -k[x.xe-x] Potassium concentration in mol.l-1. Mandatory -t[x.xe-x] Tris concentration in mol.l-1. The Tri+ concentration is about half of total Tris concentration Mandatory -G[x.xe-x] Magnesium concentration in mol.l-1. Mandatory -O[XXXXXX] Name of an output file (the name can be omitted) -P[x.xe-x] Concentration of single strand nucleic acid in mol.l-1. Mandatory -p Return path where to find the calorimetric tables -q Quiet. Switch off interactive correction of parameters -S[XXXXXXXXXX] Nucleic acid sequence, mandatory -T[XXX] Threshold for approximative computation -v Switch ON the verbose mode, issuing lot more info (if already ON, switch if OFF). Default is OFF -V Print the version number -x Force to compute an approximative tm More information is available in the user-guide. Type `man melting' to access it, or consult one of the melting.xxx files, where xxx states for lat1 (isolatin1 text), ps (postscript), pdf or html.